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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
scarabgenomics/pSX2 Expression Vector/P-3330
产品编号:P-3330
市  场 价:¥0.00
场      地:美国(厂家直采)
产品分类: 蛋白类>>其它酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:待定
品      牌: scarabgenomics
公      司:scarabgenomics
公司分类:
scarabgenomics/pSX2 Expression Vector/P-3330
商品介绍

Background

ScarabXpress®-2 system, our second-generation protein expression system, consists of the pSX2 expression plasmid and ANY Clean Genome® E. coli host strain. In side by side comparisons, the ScarabXpress®-2 system protein expression system gave up to 36x higher yields than standard expression vectors with wild-type E. coli strains

Figures

Figure 1. pSX2 Expression Plasmid Map. Figure 2. 3000-Fold Induction Range. IPTG Titration of pSX2-GFP, intensity was measured, replicates were averaged and graphed in average fluorescent units. Figure 3. ScarabXpress2 Produces ≤ 36x More Protein Than BL21(DE3). Figure 4. More Consistent Fermentations With ScarabXpress®-2

Specifications

Kit Components pSX2 Expression Vector, 10 μg Quality Control The vector is confirmed to be IS-free and for functionality. Storage Conditions Store at ≤ -70°C. Do not store in a frost-free freezer.

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Support

Product Manuals pSX2 Expression Vector

Patents & Disclaimers

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.

品牌介绍

Scarab Genomics,LLC成立于2002年,目的是将清洁基因组 ®多重缺失系列(MDS)大肠杆菌商业化,这是由麦迪逊威斯康星大学的Fred Blattner博士的研究产生的。金龟子基因组学已在威斯康星州校友研究基金会获得了专利授权的全球范围内的简化基因组技术。

通过删除超过20%的K-12基因组,对CleanGenome®E.colí进行了生物工程改造。使用合成生物学方法,进行了一系列精确的删除,包括消除了非必需基因,重组基因和移动DNA以及隐性有毒基因。减少基因组可以优化这些大肠杆菌菌株的生产,从而提高遗传稳定性和代谢效率。从常规克隆到大规模生产生物制药,CleanGenome®E.colí是广泛应用的首选菌株。

清洁Genome® 大肠杆菌菌株的 好处

  • 减少的宿主介导的重组可克隆出“不可克隆的”

  • 缺少IS元素可维持克隆完整性

  • 富媒体和最少媒体的强劲增长可带来高产量

  • 去除腐皮防止过早溶解

  • 发酵罐高密度生长

  • 经过验证的基因组减少

与传统的常用大肠杆菌宿主相比,CleanGenome®菌株在实验室克隆,质粒DNA产生和重组蛋白表达方面具有许多独特的优势圣甲虫的MDS菌株的特殊优势对于生物治疗药物的生产尤其重要。带有附加基因组缺失的MDS菌株已经制成,目前正在测试中。



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