To simplify the use of minimal medium, Scarab Genomics offers a two component kit consisting of 10X Modified Korz Medium and a separate 50X Magnesium Sulfate solution. The concentrated minimal medium and associated Magnesium Sulfate solution are diluted to create a 1X medium. A carbon source must then be added to support cell growth. We would recommend the equivalent of 0.2% glucose. The diluted 1X medium (with carbon source and appropriate antibiotics) is used for expression optimization in shake flasks. The same medium can also serves as the “batch” phase medium in fed-batch fermentations. Typically, the level of the carbon source would be adjusted to a higher level than that used in the shake flask e.g. when using glucose, the glucose level would be increased to 0.5%. Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Modified Korz Minimal Medium has been extensively tested with the Scarab Clean Genome® Strains to verify its ability to support cell growth and the production of recombinant protein. Korz minimal medium was originally designed for high density fed-batch fermentation of E. coli (Korz et al. 1995). The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements. The user needs to supply the carbon source. The same base medium used for optimizing expression in shake flasks can also be used for fed-batch fermentation, thereby providing continuity between the two processes. In fed-batch fermentations, the same medium is simply supplemented with higher carbon source content.
Kit Components
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10X Modified Korz Medium | 100 ml (D-0710-100) | 1000 ml (D-0710-1L) |
50X Magnesium Sulfate | 20 ml (D-0710-100M) | 200 ml (D-0710-1LM) |
White Glove IS Detection Kit MDS™42 Chemically Competent Cell Kit MDS™42 ΔrecA Chemically Competent Cell Kit MDS™42 ΔrecA Blue Chemically Competent Cell Kit MDS™42 Combination Package Chemically Competent Cell Kit ScarabXpress® T7 lac Chemically Competent Cell Kit MDS™42 Electrocompetent Cell Kit MDS™42 ΔrecA Electrocompetent Cell Kit MDS™42 ΔrecA Blue Electrocompetent Cell Kit MDS™42 ΔrecA trfA Electrocompetent Cell Kit MDS™42 ΔrecA trfA Blue Electrocompetent Cell Kit MDS™42 Combination Package Electrocompetent Cell Kit
Product Manuals 10X Modified Korz Medium Kit Papers
Products are sold for non-commercial use only, under Scarab Genomics limited use label license: Limited Label Use.Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications. Clean Genome® is a registered trademark of Scarab Genomics, LLC.
Scarab Genomics,LLC成立于2002年,目的是将清洁基因组 ®多重缺失系列(MDS)大肠杆菌商业化,这是由麦迪逊威斯康星大学的Fred Blattner博士的研究产生的。金龟子基因组学已在威斯康星州校友研究基金会获得了专利授权的全球范围内的简化基因组技术。
通过删除超过20%的K-12基因组,对CleanGenome®E.colí进行了生物工程改造。使用合成生物学方法,进行了一系列精确的删除,包括消除了非必需基因,重组基因和移动DNA以及隐性有毒基因。减少基因组可以优化这些大肠杆菌菌株的生产,从而提高遗传稳定性和代谢效率。从常规克隆到大规模生产生物制药,CleanGenome®E.colí是广泛应用的首选菌株。
清洁Genome® 大肠杆菌菌株的 好处
减少的宿主介导的重组可克隆出“不可克隆的”
缺少IS元素可维持克隆完整性
富媒体和最少媒体的强劲增长可带来高产量
去除腐皮防止过早溶解
发酵罐高密度生长
经过验证的基因组减少
与传统的常用大肠杆菌宿主相比,CleanGenome®菌株在实验室克隆,质粒DNA产生和重组蛋白表达方面具有许多独特的优势。圣甲虫的MDS菌株的特殊优势对于生物治疗药物的生产尤其重要。带有附加基因组缺失的MDS菌株已经制成,目前正在测试中。